Anti-Bax Rabbit pAb
   

Anti-Bax Rabbit pAb

Antibodies Oncology
AO-03-GB114122-50
BCL2L4,114122,Bax,BAX,BCL2 associated X protein,Apoptosis regulator BAX,BCL2 associated X,Bcl2-L-4,Bax-protein,Bcl-2-Like Protein 4,GB114122,Apoptosis regulator Bax,GB11007,GB11007-1
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Product Information


Protein full name

Apoptosis regulator Bax

Synonyms

BAX, BCL2L4, BCL2 associated X protein, BCL2 associated X, Apoptosis regulator BAX, Bcl-2-Like Protein 4,Bcl2-L-4, Bax-protein, apoptosis regulator

Uniprot ID

Q07812

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Bax

Isotype

IgG

Predicted MW./Observed MW.

21 kDa / 21 kDa

Purity

Affinity purification

Subcellular location

Mitochondrion, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 400-1: 800 spleen
IHC/IF Human, Mouse, Rat 1: 400-1: 800 kidney, lung, liver



Background

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.



Images

01Iu4FkTSV3PWyv.jpg

 WB analysis of Bax(GB114122).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

0273KXCAdCADHRZ.jpg

 IHC analysis of Bax(GB114122).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03L0KwPg8xIBLQG.jpg

 IHC analysis of Bax(GB114122).
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04jS0rIqdLTz4Px.jpg

 IHC analysis of Bax(GB114122).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05Zkwa5IriGxqbx.jpg

 IHC analysis of Bax(GB114122).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06oUGAYhjp9wP7Q.jpg

 IHC analysis of Bax(GB114122).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

0777wyZe9TodvGG.jpg

 IHC analysis of Bax(GB114122).
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

08Ls1rAIHCVWKpD.jpg

 IHC analysis of Bax(GB114122).
Sample: Mouse colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

09snn3O6ZvpMZCb.jpg

 IHC analysis of Bax(GB114122).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

10HdzsvALZ48pln.jpg

 IHC analysis of Bax(GB114122).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114122-50
: 10 Items
Hurry! only 10 items left in stock.

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