Anti-NOL3 Rabbit pAb

Anti-NOL3 Rabbit pAb

AO-03-GB113847-50
NOL-3,NOP30,NOL 3,Nucleolar protein of 30 kDa,NOP,GB113847,ARC,NOL3,113847,MYP,Muscle enriched cytoplasmic protein,CARD2,MYC,Apoptosis repressor with CARD,Nucleolar protein 3
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Product Information


Protein full name

Nucleolar protein 3

Synonyms

Apoptosis repressor with CARD, ARC, CARD2, MYP, NOL3, NOP, NOP30, Nucleolar protein of 30 kDa, Muscle enriched cytoplasmic protein, MYC

Uniprot ID

Q9D1X0

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse NOL3

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Membrane, Mitochondrion




Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 900-1: 1800 pancreas, heart, cerebellum




Background

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene.




Images

0198Lmnah85Niye.jpg

IHC analysis of NOL3(GB113847).
Sample: Mouse pancrease, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02stMjAaws7WhPG.jpg

IHC analysis of NOL3(GB113847).
Sample: Rat pancreas, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

031h8NPtBcnNRW0.jpg

IF analysis of NOL3(GB113847)red.
Sample: Mouse cerebellum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04rrpEfg6bJIlTQ.jpg

IF analysis of NOL3(GB113847)red.
Sample: Mouse pancreas, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05GAa7zYLSBNFms.jpg

IF analysis of NOL3(GB113847)red.
Sample: Rat pancreas, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.




Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


: AO-03-GB113847-50
: 10 Items
Hurry! only 10 items left in stock.

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