Anti-Bcl-2 Mouse mAb

Anti-Bcl-2 Mouse mAb

Antibodies Oncology
AO-03-GB124830-100
BCL2,Bcl-2,GB124830100,PPP1R50,124830,GB124830,apoptosis regulator,Bcl2,B-cell CLL/lymphoma 2,Bcl 2,Apoptosis regulator Bcl-2,GB13439
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Product Information


Protein full name

Apoptosis regulator Bcl-2

Synonyms

BCL2, Bcl-2, PPP1R50, B-cell CLL/lymphoma 2, apoptosis regulator

Uniprot ID

P10415

Immunogen

Recombinant protein corresponding to Human Bcl-2

Isotype

IgG1, κ

Clone Number

4E3F10

Predicted MW./Observed MW.

26 kDa / 26 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 spleen



Background

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells . Regulates cell death by controlling the mitochondrial membrane permeability . Appears to function in a feedback loop system with caspases . Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release .



Images

01SV55Px54JwlI8.jpg

 WB analysis of Bcl-2(GB124830).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

02PTStOGNyxQqqr.jpg

 WB analysis of Bcl-2(GB124830).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB124830-100
: 10 Items
Hurry! only 10 items left in stock.

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