Product Information
|
Protein full name |
Diablo homolog, mitochondrial |
|
Synonyms |
Diablo, DIABLO, DFNA64, SMAC, Diablo, Diablo homolog, diablo IAP-binding mitochondrial protein, Smac, diablo homolog (Drosophila),mitochondrial, DIABLO S, SMAC3 |
|
Uniprot ID |
Q9JIQ3 |
|
Immunogen |
Recombinant protein corresponding to Mouse Smac |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
27 kDa / 21 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Mitochondrion |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, brain, kidney |
| ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa, NIH/3T3 |
Background
Diablo homolog (DIABLO) is a mitochondrial protein encoded by the DIABLO gene. SMAC is a mitochondrial protein that promotes cytochrome c- and TNF receptor-dependent activation of apoptosis by inhibiting the effect of IAP–a protein that negatively regulates apoptosis, or programmed cell death. SMAC is normally a mitochondrial protein localized to the mitochondrial intermembrane space, but it enters the cytosol when cells undergo apoptosis.
Images
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WB analysis of Smac/Diablo(GB11896). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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ICC analysis of Smac/Diablo(GB11896)green. Sample: Hela, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
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ICC analysis of Smac/Diablo(GB11896)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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