Anti-Serine/threonine-protein kinase 4/MST-1 Rabbit pAb

AO-03-GB11858-100
Stk4,Anti -Serine/threonine-protein kinase 4 / MST-1,NF15S2,tk-4,HGFL,kinase4,kinase-4,GB11858,tk4,MST 1,threonine protein,tk 4,threonineprotein,MST1,D3F15S2,MSP,GB11858100,DNF15S2,11858,Macrophage stimulating 1,M,Serine/threonine-protein kinase 4 / MST-1,Serine/threonine-protein kinase 4
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Product Information


Protein full name

Serine/threonine-protein kinase 4

Synonyms

Stk4, D3F15S2, DNF15S2, HGFL, MSP, NF15S2, Macrophage stimulating 1, MST1

Uniprot ID

Q9JI11

Immunogen

Recombinant protein corresponding to Mouse MST1

Isotype

IgG

Predicted MW./Observed MW.

56 kDa / 56 kDa

Purity

Affinity purification

Subcellular location

Nucleus




Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 1500 spleen, liver, lung
IHC/IF Human 1: 400-1: 2000 colon, gastric cancer, lymph node




Background

The protein encoded by this gene contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.




Images

013F1uU8rUqgaZe.jpg

WB analysis of Serine/threonine-protein kinase 4/MST-1(GB11858).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02BPeCx4nL7VFH6.jpg

IHC analysis of Serine/threonine-protein kinase 4/MST-1(GB11858).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

038F3dsnrMlZsT2.jpg

IHC analysis of Serine/threonine-protein kinase 4/MST-1(GB11858).
Sample: Human gastric cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04WxpWPq5Zcpd4D.jpg

IHC analysis of Serine/threonine-protein kinase 4/MST-1(GB11858).
Sample: Human lymph node, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


: AO-03-GB11858-100
: 10 Items
Hurry! only 10 items left in stock.

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