Anti-Carma 1/CARD11 Rabbit pAb
   

Anti-Carma 1/CARD11 Rabbit pAb

Antibodies Oncology
AO-03-GB11828-100
CARMA1,BIMP3,Card11,PPBL,GB11828100,CARD-11,C 1,Carma 1 / CARD11,Carma-1,Caspase recruitment domain family member 11,CARD11,IMD11A,C1,Carma1,BENTA,C-1,CARD 11,11828,IMD11,GB11828,Caspase recruitment domain-containing protein 11
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Product Information


Protein full name

Caspase recruitment domain-containing protein 11

Synonyms

Card11, CARD11, BENTA, BIMP3, CARMA1, IMD11, PPBL, Caspase recruitment domain family member 11, IMD11A

Uniprot ID

Q8CIS0

Immunogen

Recombinant protein corresponding to Mouse CARD11

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 150-1: 600 brain, colon, cerebellum



Background

Caspase recruitment domain-containing protein 11 is a protein encoded by the CARD11 gene. The protein belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein.



Images

01OObvyzDHqeTHh.jpg

 IHC analysis of Carma 1/CARD11(GB11828).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02b8KAbbb3MQyV3.jpg

 IHC analysis of Carma 1/CARD11(GB11828).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03ZhJ7R5Cq6W2K9.jpg

 IHC analysis of Carma 1/CARD11(GB11828).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

046vn4wJNNp5J3n.jpg

 IHC analysis of Carma 1/CARD11(GB11828).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05ompTs7b1P2iOz.jpg

 IHC analysis of Carma 1/CARD11(GB11828).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06I32BQblEfOitb.jpg

 IF analysis of Carma 1/CARD11(GB11828)red.
Sample: Mouse epencephalon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07fhFrRqSxD0xKZ.jpg

 IF analysis of Carma 1/CARD11(GB11828)red.
Sample: Rat epencephalon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11828-100
: 10 Items
Hurry! only 10 items left in stock.

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