Recombinant Anti-NF-κB p65 antibody (Rabbit mAb) - 100 µL
   

Recombinant Anti-NF-κB p65 antibody (Rabbit mAb) - 100 µL

Antibodies-Oncology
AO-03-GB15997-100
  • Specification: 100 µL
  • Applications: WB | IHC/IF
  • Species: H,M,R | H,M,R
  • Dilution: 1: 1000-1: 2000 | 1: 2500-1: 10000
  • Positive tissue: Hela, Jurkat, lung | colon cancer, kindey, small intestine
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€429.00
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Product Information

Protein full name

Transcription factor p65

Synonyms

RELA, NFKB3, p65, RELA proto-oncogene, NF-kB subunit

Uniprot ID

Q04206

Immunogen

Recombinant protein corresponding to Human Nuclear factor NF-kappa-B p65 subunit

Isotype

IgG, κ

Clone Number

SB327

Predicted MW./Observed MW.

65 kDa / 65 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 1000-1: 2000Hela, Jurkat, lung
IHC/IFHuman, Mouse, Rat1: 2500-1: 10000colon cancer, kidney, small intestine

Background

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.

Images

01iRfZvqtiWlifN.jpg

 WB analysis of NF-κB p65(GB15997).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02mydx7SBVOJK2y.JPG

 WB analysis of NF-κB p65(GB15997).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03sq18XXCdUJFkA.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

0454OHp1pTYUFbO.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05XtvpiJTKl7T7s.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

06b7H2f4VhuU7aY.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

07naOIY7W39xOoZ.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

08loYWQZtc8njcO.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Mouse lung injury, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

09690grnqzDJaDd.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

10EH8vXuHCpJM94.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

11VQA2dLuRhgWsr.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Rat lung injury, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

12Rquweyk2Hjnwn.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Rat lung injury, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

13oAH09UVNHgD4J.jpg

 IHC analysis of NF-κB p65(GB15997).
Sample: Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

14dK7mPVuHdOauw.jpg

 IF analysis of NF-κB p65(GB15997)red.
Sample: Mouse kindey, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

15tofCYolSh7KtX.jpg

 IF analysis of NF-κB p65(GB15997)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

16zirokaIzRiBtl.jpg

 IF analysis of NF-κB p65(GB15997)red.
Sample: Rat kindey, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

17ib3qrYD5JoyMn.jpg

 IF analysis of NF-κB p65(GB15997)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

18VtL2ItjhCjZAB.jpg

 IF analysis of NF-κB p65(GB15997)red.
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB15997-100
: 10 Items
Hurry! only 10 items left in stock.

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