Anti-NF-κB p65 Mouse mAb - 100 µL
   

Anti-NF-κB p65 Mouse mAb - 100 µL

Antibodies-Oncology
AO-03-GB12997-100
  • Specification: 100 µL
  • Applications: WB | IHC/IF
  • Species: H,M,R | M,R
  • Dilution: 1: 500-1: 1000 | 1: 500-1: 1000
  • Positive tissue: HeLa, C6, thymus | Inflammatory model induced by lps(6h) colon, Inflammatory model induced by lps(24h) spleen
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€429.00
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Product Information

Protein full name

Transcription factor p65

Synonyms

RELA, NFKB3, p65, RELA proto-oncogene, NF-kB subunit

Uniprot ID

Q04207

Immunogen

Recombinant protein corresponding to Mouse Nuclear factor NF-kappa-B p65 subunit

Isotype

IgG2b, κ

Clone Number

7B9B4

Predicted MW./Observed MW.

65 kDa / 65 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000HeLa, C6, thymus
IHC/IFMouse, Rat1: 500-1: 1000Inflammatory model induced by lps(6h) colon, Inflammatory model induced by lps(24h) spleen, subcutaneous nude-mouse model of 4T1 cell

Background

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.

Images

011Zd5CyXKcbeoc.jpg

 WB analysis of NF-κB p65(GB12997).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

02qNF85ZV1Muygh.jpg

 IHC analysis of NF-κB p65(GB12997).
Sample: Mouse Inflammatory model induced by lps(24h) spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 95°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

03oFNPt4iVzDZYY.jpg

 IHC analysis of NF-κB p65(GB12997).
Sample: Rat Inflammatory model induced by lps(6h) colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 95°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

04mTojFMivrnyIU.jpg

 IHC analysis of NF-κB p65(GB12997).
Sample: Rat Inflammatory model induced by lps(24h) spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 95°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

0544qZSUaLkZSE4.jpg

 IHC analysis of NF-κB p65(GB12997).
Sample: Mouse Inflammatory model induced by lps(6h) colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 95°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

06yG0faGZ8HgWLK.jpg

 IF analysis of NF-κB p65(GB12997)red.
Sample: Mouse subcutaneous nude-mouse model of 4T1 cell, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 95°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

07chqyBZ9DB8mlF.jpg

 IF analysis of NF-κB p65(GB12997)red.
Sample: Rat Inflammatory model induced by lps(24h) spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 95°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB12997-100
: 10 Items
Hurry! only 10 items left in stock.

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