Recombinant Anti-IKB alpha antibody (Mouse mAb) - 50 µL

Recombinant Anti-IKB alpha antibody (Mouse mAb) - 50 µL

Antibodies-Oncology
AO-03-GB151509-50
  • Specification: 50 µL
  • Applications: WB
  • Species: H,M,R
  • Dilution: 1:1000-1:2000
  • Positive tissue: HeLa+Calyculin A,NIH/3T3+Calyculin A, HeLa, NIH3T3
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Product Information

Protein full name

NF-kappa-B inhibitor alpha

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Uniprot ID

P25963

Immunogen

KLH conjugated Synthetic peptide corresponding to Human IKBα

Isotype

IgG1, κ

Clone Number

SB90

Predicted MW./Observed MW.

36 kDa / 36 kDa

Purity

Affinity purification

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 1000-1: 2000HeLa+Calyculin A,NIH/3T3+Calyculin A,C6+Calyculin A

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Images

01g8MLIYapIHiRF.jpg

 WB analysis of IKB alpha(GB151509).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB151509-50
: 10 Items
Hurry! only 10 items left in stock.

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