Product Information
Protein full name | Rhotekin-2 |
Synonyms | PLEKHK1, rhotekin 2, RTKN2 |
Uniprot ID | Q14B46 |
Immunogen | Recombinant protein corresponding to Mouse RTKN2 |
Isotype | IgG |
Predicted MW./Observed MW. | 67 kDa / 70 kDa |
Purity | Affinity purification |
Subcellular location | Cell membrane, Cytoplasm, Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse | 1: 500-1: 800 | lymph node |
| IHC/IF | Human | 1: 600-1: 1200 | lung |
Background
May play an important role in lymphopoiesis. {ECO:0000250}.
Images
| WB analysis of RTKN2(GB114538). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| IHC analysis of RTKN2(GB114538). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IF analysis of RTKN2(GB114538)red. Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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