Anti-MyD88 Rabbit pAb - 50 µL
   

Anti-MyD88 Rabbit pAb - 50 µL

Antibodies-Oncology
AO-03-GB111554-50
  • Specification: 50 µL
  • Applications: WB | IHC/IF
  • Species: H,M,R | H,M,R
  • Dilution: 1: 500-1: 1000 | 1: 1200-1: 2400
  • Positive tissue: kidney, liver, lung | small intestine, lung, pneumonia
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€299.00
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Product Information

Protein full name

Myeloid differentiation primary response protein MyD88

Synonyms

MYD88, MYD88D, mutant myeloid differentiation primary response 88, myeloid differentiation primary response gene (88)

Uniprot ID

Q99836

Immunogen

KLH conjugated Synthetic peptide corresponding to Human MyD88

Isotype

IgG

Predicted MW./Observed MW.

33 kDa / 33 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000kidney, liver, lung
IHC/IFHuman, Mouse, Rat1: 1200-1: 2400spleen, lung

Background

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS, which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis.

Images

012ysKnka1NdFur.jpg

WB analysis of MyD88(GB111554).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02G4mMiYxM5pF7U.jpg

WB analysis of MyD88(GB111554).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03sd8gGviA3KyUL.jpg

WB analysis of MyD88(GB111554).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

04KhrHRDTOLT9FQ.jpg

IHC analysis of MyD88(GB111554).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1200, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05ULHgI3WTieshU.jpg

IHC analysis of MyD88(GB111554).
Sample: Human small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1200, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06SUCuuNvRIc73k.jpg

IHC analysis of MyD88(GB111554).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1200, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

07IOSl2hU2xZnZd.jpg

IHC analysis of MyD88(GB111554).
Sample: Rat pneumonia, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1200, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

StorageStore at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB111554-50
: 10 Items
Hurry! only 10 items left in stock.

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