Anti-IKB beta Rabbit pAb - 100 µL

Anti-IKB beta Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB114064-100
  • Specification: 100 µL
  • Applications: WB
  • Species: H,M,R
  • Dilution: 1: 1000-1: 2000
  • Positive tissue: heart, testis
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Product Information

Protein full name

NF-kappa-B inhibitor beta

Synonyms

I kappa B beta, IkappaBbeta, IkB B, IkB beta, IKBB, NF kappa B inhibitor beta, NF kappa BIB, NFKBIB, TR interacting protein 9, TRIP 9, TRIP9, IKBB, IkB-beta, NFKB inhibitor beta

Uniprot ID

Q60778

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse IKB-beta

Isotype

IgG

Predicted MW./Observed MW.

38 kDa / 45-48 kDa

Purity

Affinity purification

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 1000-1: 2000heart, testis

Background

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Images

01o1Ldablkjm2EQ.jpg

 WB analysis of IKB beta(GB114064).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02TBVNHEXJzWk7H.jpg

 WB analysis of IKB beta(GB114064).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB114064-100
: 10 Items
Hurry! only 10 items left in stock.

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