Anti-Nucleoporin p62/NUP62 Rabbit pAb - 100 µL

Anti-Nucleoporin p62/NUP62 Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB111998-100
  • Specification: 100 µL
  • Applications: WB | IHC/IF
  • Species: H,M,R | M,R
  • Dilution: 1: 500-1: 1000 | 1: 500-1: 1500
  • Positive tissue: testis, colon, liver | brain, rectum
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Product Information

Protein full name

Nuclear pore glycoprotein p62

Synonyms

62 kDa nucleoporin, Nucleoporin Nup62, Nup62, IBSN, p62, SNDI

Uniprot ID

Q63850

Immunogen

Recombinant protein corresponding to Mouse Nucleoporin p62

Isotype

IgG

Predicted MW./Observed MW.

53 kDa / 72 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000testis, colon, liver
IHC/IFMouse, Rat1: 500-1: 1500brain, rectum

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals.

Images

015cpUXuPAPZPyG.jpg

 WB analysis of Nucleoporin p62/NUP62(GB111998).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02LlpDbegVIkKsm.jpg

 WB analysis of Nucleoporin p62/NUP62(GB111998).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03B1Y8m6cnBcp5d.jpg

 IHC analysis of Nucleoporin p62/NUP62(GB111998).
Sample: Mouse rectum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 650, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB111998-100
: 10 Items
Hurry! only 10 items left in stock.

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