Product Information
Protein full name | Transcription factor RelB |
Synonyms | Reticuloendotheliosis viral oncogene homolog B, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, IREL, v rel avian reticuloendotheliosis viral oncogene homolog, RELB, I-REL, IREL, REL-B, RELB proto-oncogene, NF-kB subunit |
Uniprot ID | Q04863 |
Immunogen | Recombinant protein corresponding to Mouse Rel B |
Isotype | IgG |
Predicted MW./Observed MW. | 60 kDa / 70 kDa |
Purity | Affinity purification |
Subcellular location | Nucleus, Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, colon |
| IHC/IF | Human, Mouse | 1: 1000-1: 2000 | colon, colon cancer, small intestine |
Background
RelB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF- kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.
Images
| WB analysis of Rel B(GB11985).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| IHC analysis of Rel B(GB11985).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Rel B(GB11985).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Rel B(GB11985).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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