Anti-NFkB p100/NFKB2 Rabbit pAb - 100 µL

Anti-NFkB p100/NFKB2 Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB11651-100
  • Specification: 100 µL
  • Applications: WB
  • Species: H
  • Dilution: 1: 1000-1: 2000
  • Positive tissue: Hela, Jurkat, MCF7
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Product Information

Protein full name

Nuclear factor NF-kappa-B p100 subunit

Synonyms

DNA-binding factor KBF2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Nuclear factor NF-kappa-B p52 subunit, Nfkb2, Lyt10, H2TF1, NFKB2, CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p105, p52, p100, p49/p100

Uniprot ID

Q00653

Immunogen

Recombinant protein corresponding to Human Nuclear factor NF-kappa-B p100 subunit

Isotype

IgG

Predicted MW./Observed MW.

97 kDa / 100 kDa

Purity

Affinity purification

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman1: 1000-1: 2000HeLa, Jurkat, MCF7

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing.

Images

01bp9jQgJMuv4VV.jpg

 WB analysis of NFkB p100/NFKB2(GB11651).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB11651-100
: 10 Items
Hurry! only 10 items left in stock.

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