Anti-IKK alpha Rabbit pAb - 100 µL

Anti-IKK alpha Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB11923-100
  • Specification: 100 µL
  • Applications: WB
  • Species: H,M,R
  • Dilution: 1: 500-1: 1000
  • Positive tissue: thymus, testis
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Product Information

Protein full name

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Synonyms

Chuk, CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, Conserved helix-loop-helix ubiquitous kinase, Component of inhibitor of nuclear factor kappa B kinase complex

Uniprot ID

Q60680

Immunogen

Recombinant protein corresponding to Mouse IKKα

Isotype

IgG

Predicted MW./Observed MW.

85 kDa / 85 kDa

Purity

Affinity purification

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000thymus, testis

Background

IKK-α is a member of the serine/threonine protein kinase family and forms a complex in the cell with IKK-βand NEMO. NF-κB transcription factors are normally held in an inactive state by the inhibitory proteins IκBs. IKK-α and IKK-β phosphorylate the IκB proteins, marking them for degradation via ubiquitination and allowing NF-κB transcription factors to go into the nucleus. Once activated, NF-κB transcription factors regulate genes that are implicated in many important cellular processes, including immune response, inflammation, cell death, and cell proliferation.

Images

01Bh1Ke9vY2W5fG.jpg

 WB analysis of IKK alpha(GB11923).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02Da7UqOJPA9pvv.jpg

 WB analysis of IKK alpha(GB11923).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB11923-100
: 10 Items
Hurry! only 10 items left in stock.

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