Français
English
Recombinant Anti-beta III Tubulin antibody (Mouse mAb), Primary Antibody
AO-03-GB15139-50
Product Information
|
Protein full name |
Tubulin beta-3 chain |
|
Synonyms |
TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin, TUJ-1,TUJ 1, TUJ1, tuj1 |
|
Immunogen |
Recombinant protein corresponding to Mouse beta III Tubulin |
|
Uniprot ID |
Q9ERD7 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, Cytoskeleton |
|
Predicted MW. / Observed MW. |
55kDa / 55kDa |
Applications
|
WB |
Human, Mouse, Rat |
1: 1000-1:2000 |
brain, cerebral cortex |
|
IHC/IF |
Human, Mouse, Rat, Monkey |
1: 600-1: 1200 |
Cerebellum, brain |
Background
Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.
Images
|
|
WB analysis of beta III Tubulin(GB15139). |
|
|
IF analysis of beta III Tubulin(GB15139)red. Sample: Mouse cerebellum, 4% PFA (G1101) 12-24h. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
|
|
IHC analysis of beta III Tubulin(GB15139). Sample: Monkey brain, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
: AO-03-GB15139-50
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package