Anti-FOXP3 Rabbit pAb, Primary Antibody

Anti-FOXP3 Rabbit pAb, Primary Antibody

AO-03-GB112325-50

Anti-FOXP3 Rabbit pAb

Volume : 50 µL
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Product Information

Protein full name

Forkhead box protein P3

Synonyms

Scurfin, FOXP3, IPEX, JM2, AIID, DIETER, Immunodeficiency polyendocrinopathy enteropathy X linked, PIDX, XPID, forkhead box P3

Immunogen

KLH conjugated Synthetic peptide corresponding to Human FOXP3

Uniprot ID

Q9BZS1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Predicted MW. / Observed MW.

47 kDa / 47 kDa

Applications

WB

Human, Mouse, Rat

1: 500-1: 1000

lymph node, thymus

IHC/IF

Human, Mouse, Rat

1: 500-1: 1800

lymph node, thymus

Background

FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells.Regulatory T cells generally turn the immune response down. In cancer, an excess of regulatory T cell activity can prevent the immune system from destroying cancer cells. In autoimmune disease, a deficiency of regulatory T cell activity can allow other autoimmune cells to attack the body's own tissues.

Images

图片 4.png

WB analysis of FOXP3 (GB112325).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

Picture 10.png

IF analysis of FOXP3 (GB112325).
Sample: Mouse lymph node (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

Picture 11.png

IF analysis of FOXP3 (GB112325).
Sample: Rat thymus (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
: AO-03-GB112325-50
: 10 Produits
Hurry! only 10 items left in stock.

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