Anti-MLF1 Interacting Protein/PBIP1 Rabbit pAb
   

Anti-MLF1 Interacting Protein/PBIP1 Rabbit pAb

Antibodies Oncology
AO-03-GB114849-50
CENP U,CENP50,PBIP-1,114849,PBIP1,HHV8 LNAIP1,MLF1IP,centromere protein of 50 kDa,KLIP1,Polo-box-interacting protein 1MLF1-interacting protein,MLF-1,CENPU50,ICEN24,PBIP 1,GB114849,MLF 1,CENP-50,MLF1IP1,LNAIP 1,MLF1 Interacting Protein/PBIP1,KSHV LNAIP1,Centromere protein U,action
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Product Information


Protein full name

Centromere protein U

Synonyms

CENP U, CENP-50, CENP50, CENPU50, centromere protein of 50 kDa, HHV8 LNAIP1, ICEN24, KLIP1, LNAIP 1, KSHV LNAIP1, MLF1IP, MLF1IP1, PBIP1, Polo-box-interacting protein 1MLF1-interacting protein

Uniprot ID

Q8C4M7

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MLF1 Interacting Protein/PBIP1

Isotype

IgG

Predicted MW./Observed MW.

46 kDa / 45 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Chromosome



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 heart,kidney,heart
IHC/IF Human, Mouse, Rat 1: 300-1: 900 colon cancer, testis, colon, liver



Background

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.



Images

01hp2pfFJncNo0n.jpg

 WB analysis of MLF1 Interacting Protein/PBIP1(GB114849).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

0263Prs74wglQaW.jpg

 IHC analysis of MLF1 Interacting Protein/PBIP1(GB114849).
Sample: Mouse Colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03OV9gTWFtLADJ2.jpg

 IHC analysis of MLF1 Interacting Protein/PBIP1(GB114849).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

043SbSBM2A6D7XT.jpg

 IHC analysis of MLF1 Interacting Protein/PBIP1(GB114849).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05wAABewjTLRHyq.jpg

 IHC analysis of MLF1 Interacting Protein/PBIP1(GB114849).
Sample: Rat Colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

0611OFsqnBrVxpQ.jpg

 IHC analysis of MLF1 Interacting Protein/PBIP1(GB114849).
Sample: Human liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

07nw198rH0ro4Q3.jpg

 IHC analysis of MLF1 Interacting Protein/PBIP1(GB114849).
Sample: Human Colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114849-50
: 10 Produits
Hurry! only 10 items left in stock.

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