Anti-Caprin-1 Rabbit pAb

Anti-Caprin-1 Rabbit pAb

Antibodies Oncology
AO-03-GB113530-50
GB113530,GPIAP1,Caprin1,GPI anchored protein p137,ytoplasmic activation- and proliferation-associated protein 1,GPI-p137,113530,CAPRIN1,Caprin-1,caprin 1,G5E5,GB113530100,p137GPI,RNG105,Gpiap,GPIP137,M11S1,Caprin 1,Caprin-1
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Product Information


Protein full name

Caprin-1

Synonyms

caprin 1, CAPRIN1, GPI anchored protein p137, GPI-p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105, G5E5, Gpiap, ytoplasmic activation- and proliferation-associated protein 1

Uniprot ID

Q60865

Immunogen

Recombinant protein corresponding to Mouse Caprin-1

Isotype

IgG

Predicted MW./Observed MW.

78 kDa / 116 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse 1: 1000-1: 1500 testis
ICC/IF Human, Mouse 1: 100-1: 300 HeLa



Background

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.



Images

01111LKoQSlciWo.jpg

 WB analysis of Caprin-1(GB113530).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02dTsM1lBCjcPmZ.jpg

 ICC analysis of Caprin-1(GB113530)green.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB113530-50
: 10 Produits
Hurry! only 10 items left in stock.

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