Anti-ROR alpha/RORA Mouse mAb
   

Anti-ROR alpha/RORA Mouse mAb

Antibodies Oncology
AO-03-GB121300-50
Rora,ROR alpha/RORA,121300,RAR-related orphan receptor A,RORalpha,Nr1f1,Rzra,Nuclear receptor RZR-alpha,ROR-alpha,Retinoid-related orphan receptor-alpha,GB121300,Nuclear receptor subfamily 1 group F member 1,Nuclear receptor ROR-alpha
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Ajouter souhaits

Product Information


Protein full name

Nuclear receptor ROR-alpha

Synonyms

Nuclear receptor RZR-alpha, Nuclear receptor subfamily 1 group F member 1, RAR-related orphan receptor A, Retinoid-related orphan receptor-alpha, Rora, Nr1f1, Rzra

Uniprot ID

P51448

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse ROR alpha

Isotype

IgG2a, κ

Clone Number

5D7A7

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 900-1: 1800 brain, heart, kidney



Background

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways . It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'.



Images

01PApjAnKrd3n2b.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Human melanoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

02TQSiCbJgfjUyO.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Human renal cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

03PkcvZ8lEQLsFz.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

04BfPEkkC86JMct.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

05yHahL50PDef8p.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

06e1r09VvFc64e1.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

07x7XVHcy4AW0En.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

084NdQ1N34QvxmO.jpg

 IHC analysis of ROR alpha/RORA(GB121300).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 900, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB121300-50
: 10 Produits
Hurry! only 10 items left in stock.

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