Anti-TPX2 Rabbit pAb
   

Anti-TPX2 Rabbit pAb

Antibodies Oncology
AO-03-GB112703-50
TPX 2,p100,TPX-2,C20orf2,Protein fls353,C20orf1,GB112703,Differentially expressed in cancerous and non-cancerous lung cells 2,TPX2,DIL-2,HCA519,DIL2,112703,Targeting protein for Xklp2
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Ajouter souhaits

Product Information


Protein full name

Targeting protein for Xklp2

Synonyms

Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, p100, C20orf1, C20orf2, DIL2, HCA519, TPX2, Protein fls353, Tpx2, C20ORF1

Uniprot ID

Q9ULW0

Immunogen

KLH conjugated Synthetic peptide corresponding to Human TPX2

Isotype

IgG

Predicted MW./Observed MW.

86 kDa / 100 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Cytoskeleton, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 HeLa, HepG2, MCF7
IHC/IF Human, Mouse, Rat 1: 300-1: 1000 lung cancer, breast cancer, prostate



Background

TPX2 is essential for spindle pole and mitotic spindle formulation, playing a role in the S and G2 phases of mitosis. It is also critical for the function of protein kinase Aurora A. TPX2 autophosphorylates Aurora A, targeting it to the spindle apparatus and allowing it to function in eukaryotic meiotic and mitotic cell cycles. TPX2 has also been found to be highly expressed in cancer cells.



Images

01zPRO8ZPJn0EcF.jpg

 WB analysis of TPX2(GB112703).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

0271ZviKpDqVjuy.jpg

 IHC analysis of TPX2(GB112703).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03iqTRnJtv1uzlH.jpg

 IHC analysis of TPX2(GB112703).
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

042bmHI4DXzA2Mp.jpg

 IHC analysis of TPX2(GB112703).
Sample: Human prostate, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

058PwJWDjZKuUdk.jpg

 IHC analysis of TPX2(GB112703).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06xjVBWHAk57QZG.jpg

 IHC analysis of TPX2(GB112703).
Sample: Rat injured bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

073vteXfyuoqRI5.jpg

 IF analysis of TPX2(GB112703)red.
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08z5vnNJcdGn1UP.jpg

 IF analysis of TPX2(GB112703)red.
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09u5ffGHYvZHtZD.jpg

 IF analysis of TPX2(GB112703)red.
Sample: Rat breast, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112703-50
: 10 Produits
Hurry! only 10 items left in stock.

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