Anti-MID1 Rabbit pAb
   

Anti-MID1 Rabbit pAb

Antibodies Oncology
AO-03-GB112569-50
GB112569,Tripartite motif-containing protein 18,MID 1,Trim18,112569,RING finger protein Midline-1,Midin,MID1,Fxy,RING-type E3 ubiquitin transferase Midline-1,Mid1,MID-1,E3 ubiquitin-protein ligase Midline-1
check En Stock
Hurry! only 10 items left in stock.
299,00 €
HT
Quantité

Ajouter souhaits

Product Information


Protein full name

E3 ubiquitin-protein ligase Midline-1

Synonyms

Midin, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18, Mid1, Fxy, Trim18

Uniprot ID

O70583

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MID1

Isotype

IgG

Predicted MW./Observed MW.

76 kDa / 76 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Cytoskeleton



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 2000-1: 3000 heart, brain, testis, liver
IHC/IF Human 1: 600-1: 1200 tonsil



Background

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.



Images

01ukqBHhlbjB7Yk.jpg

 WB analysis of MID1(GB112569).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02APljJqev0LcIK.jpg

 WB analysis of MID1(GB112569).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03MwPB5UAceSlfK.jpg

 WB analysis of MID1(GB112569).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

04jGjxaO7b4Sh6F.jpg

 WB analysis of MID1(GB112569).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

054QccnF89P1TJO.jpg

 IHC analysis of MID1(GB112569).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112569-50
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package