Anti-NIPA Rabbit pAb

Anti-NIPA Rabbit pAb

Antibodies Oncology
AO-03-GB112191-50
Nipa,mNIPA,hNIPA,GB112191,NIPA,Zinc finger C3HC-type protein 1,Anti- NIPA Rabbit pAb,112191,Nuclear-interacting partner of anaplastic lymphoma kinase,Zc3hc1,Nuclear-interacting partner of ALK
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Product Information


Protein full name

Nuclear-interacting partner of ALK

Synonyms

Nuclear-interacting partner of anaplastic lymphoma kinase, mNIPA, Zinc finger C3HC-type protein 1, Zc3hc1, Nipa, hNIPA

Uniprot ID

Q80YV2

Immunogen

Recombinant protein corresponding to Mouse NIPA

Isotype

IgG

Predicted MW./Observed MW.

55 kDa / 55 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 A431, HeLa, U251



Background

The regulated oscillation of protein expression is an essential mechanism of cell cycle control. The SCF class of E3 ubiquitin ligases is involved in this process by targeting cell cycle regulatory proteins for degradation by the proteasome, with the F-box subunit of the SCF specifically recruiting a given substrate to the SCF core. NIPA (nuclear interaction partner of ALK) is a human F-box-containing protein that defines an SCF-type E3 ligase (SCFNIPA) controlling mitotic entry. Assembly of this SCF complex is regulated by cell-cycle-dependent phosphorylation of NIPA, which restricts substrate ubiquitination activity to interphase.



Images

01ojdP68qNElEX7.jpg

 WB analysis of NIPA(GB112191).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112191-50
: 10 Produits
Hurry! only 10 items left in stock.

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