Anti-Cyclin B1 Rabbit pAb
   

Anti-Cyclin B1 Rabbit pAb

Antibodies Oncology
AO-03-GB112098-50
Cycb1,Anti- Cyclin B1 Rabbit pAb,Cyclin-B1,Cyclin B1,Ccn-2,Ccnb1,B-1,CyclinB1,B 1,Ccnb1-rs13,CCNB,GB112098,Cycb,112098,G2/mitotic-specific cyclin-B1
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Product Information


Protein full name

G2/mitotic-specific cyclin-B1

Synonyms

CCNB, CCNB1, Cyclin B1, G2/mitotic specific cyclin B1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1, Ccnb1, CCNB

Uniprot ID

P24860

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1

Isotype

IgG

Predicted MW./Observed MW.

48 kDa / 55 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Cytoskeleton, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 testis, colon, spleen
IHC/IF Human, Mouse, Rat 1: 300-1: 3000 colon cancer, skin, colon



Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.



Images

017AhtYNq8bkCiV.jpg

 WB analysis of Cyclin B1(GB112098).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02eTz1vCR9y91KM.jpg

 WB analysis of Cyclin B1(GB112098).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03tX7aj4b2dH84m.jpg

 IHC analysis of Cyclin B1(GB112098).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04cEeeBLeS9IcDZ.jpg

 IHC analysis of Cyclin B1(GB112098).
Sample: Human skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05fjo7hiwkERBlj.jpg

 IHC analysis of Cyclin B1(GB112098).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06j45hh9fKJlouP.jpg

 IHC analysis of Cyclin B1(GB112098).
Sample: Mouse colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

07YLM2INZmskUWg.jpg

 IF analysis of Cyclin B1(GB112098)red.
Sample: Human cervical cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08LzDfp2YnedtOW.jpg

 IF analysis of Cyclin B1(GB112098)red.
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09tbk7MN4s8E31A.jpg

 IF analysis of Cyclin B1(GB112098)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

10eXPtEXCBZve8J.jpg

 IF analysis of Cyclin B1(GB112098)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

11frGh4JcSBdRXP.jpg

 IF analysis of Cyclin B1(GB112098)red.
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112098-50
: 10 Produits
Hurry! only 10 items left in stock.

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