Anti-14-3-3 alpha+beta Mouse mAb
   

Anti-14-3-3 alpha+beta Mouse mAb

Antibodies Oncology
AO-03-GB12592-50
GW128,KCIP-1,12592,tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta,GB12592,YWHAB,HS1,HEL-S-1,14-3-3 alpha + beta,β 14-3-3,YWHAA,Anti-14-3-3 alpha Mouse mAb,14-3-3 alpha + β,beta 14-3-3,14 3 3,1433,14-3-3 protein beta/alpha
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Ajouter souhaits

Product Information


Protein full name

14-3-3 protein beta/alpha

Synonyms

YWHAB, GW128, HEL-S-1, HS1, KCIP-1, YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta

Uniprot ID

Q9CQV8

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse 14-3-3 alpha + beta

Isotype

IgG2b, κ

Clone Number

4B12D11

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


ApplicationsSpeciesDilutionPositive Tissue
IHC/IFHuman, Mouse, Rat1: 500-1: 1000colon, esophagus cancer, fetal rat



Background


This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.



Images

01FBz0S46wd2Ifc.jpg                        

IHC analysis of 14-3-3 alpha+beta(GB12592).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

026x7ha5W0S2Ar1.jpg                        

IHC analysis of 14-3-3 alpha+beta(GB12592).
Sample: Human esophagus cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

03lLcKPN6it7brN.jpg                        

IF analysis of 14-3-3 alpha+beta(GB12592)red.
Sample: Human intestine cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

04ShtHCCBoyaESK.jpg                        

IF analysis of 14-3-3 alpha+beta(GB12592)red.
Sample: Mouse fetal rat, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

05S8ImmrulS860y.jpg                        

IF analysis of 14-3-3 alpha+beta(GB12592)red.
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

064qLsuyFpeahpq.jpg                        

IF analysis of 14-3-3 alpha+beta(GB12592)red.
Sample: Rat skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB12592-50
: 10 Produits
Hurry! only 10 items left in stock.

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