Anti-p70 S6 Kinase Rabbit pAb

Protein full name

Ribosomal protein S6 kinase beta-1

Synonyms

S6K-beta-1, p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K1, STK14A

Immunogen

Recombinant protein corresponding to Mouse p70 S6 Kinase

Uniprot ID

Q8BSK8

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Predicted MW./Observed MW.

51-60 kDa / 70 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

brain, testis

ICC/IF

Mouse, Rat

1: 100-1: 400

NIH-3T3

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy . RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59 kDa produced by alternative initiation.

Images

WB analysis of p70 S6 Kinase(GB111133).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

ICC analysis of p70 S6 Kinase(GB111133)red.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.