Product Information
|
Protein full name |
Cyclin-A2 |
|
Synonyms |
Cyclin-A, Ccna2, CycA, CycA2, Cyclin A2, Cyclin A, CCNA2, CCNA, CCN1 |
|
Uniprot ID |
P20248 |
|
Immunogen |
Recombinant protein corresponding to Human Cyclin A2 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
49 kDa / 55 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse | 1: 500-1: 1000 | spleen, lymph node |
| IHC/IF | Human, Mouse, Rat | 1: 250-1: 1200 | lymph, brain, lung cancer |
| ICC/IF | Human | 1: 50-1: 200 | HeLa |
Background
Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.
Images
|
|
WB analysis of Cyclin A2(GB111132). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
|
|
WB analysis of Cyclin A2(GB111132). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
|
|
IHC analysis of Cyclin A2(GB111132). Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of Cyclin A2(GB111132). Sample: Rat lymph, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of Cyclin A2(GB111132). Sample: Mouse lymph, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of Cyclin A2(GB111132). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of Cyclin A2(GB111132). Sample: Human lymph gland, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
|
|
IF analysis of Cyclin A2(GB111132)red. Sample: Mouse placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of Cyclin A2(GB111132)red. Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
ICC analysis of Cyclin A2(GB111132)green. Sample: Hela, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4℃ overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
|
|
ICC analysis of Cyclin A2(GB111132)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
Français
English
