Anti-Chk2 Mouse mAb

Anti-Chk2 Mouse mAb

Antibodies Oncology
AO-03-GB12575-50
CHEK2,CDS1,Chk2,hCds1,GB12575,LFS2,checkpoint kinase 2,Chk 2,12575,RAD53,Chk-2,PP1425,HuCds1,CHK2,Serine/ threonine-protein kinase Chk2
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Product Information


Protein full name

Serine/ threonine-protein kinase Chk2

Synonyms

CHEK2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, checkpoint kinase 2

Uniprot ID

Q9Z265

Immunogen

Recombinant protein corresponding to Mouse Chk2

Isotype

IgG1, κ

Clone Number

3C11E9

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human 1: 100-1: 1000 colon, colon cancer, stomach cancer



Background

CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2, a serine-threonine kinase. CHK2 regulates cell division, and has the ability to prevent cells from dividing too rapidly or in an uncontrolled manner, So CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2.



Images

01w1hcOusQrouFM.jpg

 IHC analysis of Chk2(GB12575).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

020A5UKAeFfSYy3.jpg

 IHC analysis of Chk2(GB12575).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

0334Jg26HgqUxbb.jpg

 IHC analysis of Chk2(GB12575).
Sample: Human stomach cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB12575-50
: 10 Produits
Hurry! only 10 items left in stock.

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