Anti-Cdc20B Rabbit pAb

Anti-Cdc20B Rabbit pAb

Antibodies Oncology
AO-03-GB115217-100
CDC-20B,G6VTS76519,115217,GB115217,CDC 20,CDC 20B,CDC-20,CDC20B,GB115217100,Cell division cycle protein 20 homolog B
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Product Information


Protein full name

Cell division cycle protein 20 homolog B

Synonyms

CDC20B, G6VTS76519

Uniprot ID

Q86Y33

Immunogen

KLH conjugated Synthetic peptide corresponding to Human CDC20B

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


ApplicationsSpeciesDilutionPositive Tissue
IHC/IFMouse, Rat1: 800-1: 2400kidney
ICC/IFHuman, Mouse1: 200-1: 800HeLa



Background


The timely destruction of key regulators through ubiquitin-mediated proteolysis ensures the orderly progression of the cell cycle. The APC (anaphase-promoting complex) is a major component of this degradation machinery and its activation is required for the execution of critical events. CDC20B has been implicated as a meiosis specific activator of APC and is required for sporulation in budding yeast.



Images

01YsqN2sJAOLzr8.jpg                        

IHC analysis of Cdc20B(GB115217).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02UAWGyaKt4TkzM.jpg                        

IHC analysis of Cdc20B(GB115217).
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 2400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03ZGqfQJPlvlQWq.jpg                        

ICC analysis of Cdc20B(GB115217)red.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB115217-100
: 10 Produits
Hurry! only 10 items left in stock.

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