Anti-YIPF4 Rabbit pAb
   

Anti-YIPF4 Rabbit pAb

Antibodies Oncology
AO-03-GB113863-100
Yip1 domain family,YIPF-4,Nbla11189,GB113863,113863,Protein YIPF4,YIP1 family member 4,FinGER4,GB113863100,member 4,YIPF4,YIPF 4,Protein YIPF4
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Ajouter souhaits

Product Information


Protein full name

Protein YIPF4

Synonyms

FinGER4, Nbla11189, Protein YIPF4, Yip1 domain family, member 4, YIP1 family member 4, YIPF4

Uniprot ID

Q8C407

Immunogen

Recombinant protein corresponding to Mouse YIPF4

Isotype

IgG

Predicted MW./Observed MW.

27 kDa / 27 kDa

Purity

Affinity purification

Subcellular location

Golgi apparatus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 spleen, placenta
IHC/IF Human, Mouse, Rat 1: 500-1: 1200 colon, duodenum, heart



Background

Involved in the maintenance of the Golgi structure.



Images

01pBk2MtHsYbjsn.jpg

 WB analysis of YIPF4(GB113863).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02sefgHkZKKXTB5.jpg

 IHC analysis of YIPF4(GB113863).
Sample: Mouse duodenum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03He9EOxjAKTyOW.jpg

 IHC analysis of YIPF4(GB113863).
Sample: Rat duodenum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04KsJo6j1gZc87k.jpg

 IF analysis of YIPF4(GB113863)red.
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05emBgRIYfr7oHT.jpg

 IF analysis of YIPF4(GB113863)red.
Sample: Mouse colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

0608cnnLeC2vjeZ.jpg

 IF analysis of YIPF4(GB113863)red.
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB113863-100
: 10 Produits
Hurry! only 10 items left in stock.

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