Product Information
|
Protein full name |
Wee1-like protein kinase 2 |
|
Synonyms |
WEE1 homolog 2 (S. pombe), Wee1 like protein kinase 1B, WEE1B, Wee1B kinase, WEE2 |
|
Uniprot ID |
Q66JT0 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse WEE2 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
62 kDa / 62 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 300-1: 600 | Jurkat, A-431, U-20S |
Background
WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque.
Images
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|
WB analysis of WEE2(GB113669). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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