Anti-Rad21 Rabbit pAb

Anti-Rad21 Rabbit pAb

Antibodies Oncology
AO-03-GB111435-100
111435,Rad 21,KIAA0078,GB111435100,NXP-1,Anti-Rad21 Rabbit pAb,NXP1,Rad-21,SCC1 homolog,Nuclear matrix protein 1,Rad21,SCC1,64-kDa carboxy-terminal product,hHR21,GB111435,HR21,Double-strand-break repair protein rad21 homolog
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Product Information


Protein full name

Double-strand-break repair protein rad21 homolog

Synonyms

hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog, 64-kDa carboxy-terminal product, Rad21, HR21, KIAA0078, NXP1, SCC1

Uniprot ID

Q61550

Immunogen

Recombinant protein corresponding to Mouse Rad21

Isotype

IgG

Predicted MW./Observed MW.

72 kDa / 75 /130 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Chromosome, centromere, Cytoplasm,cytosol



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 HeLa, THP-1, K562
ICC/IF Human 1: 100-1: 400 HeLa



Background

RAD21, also named as Nuclear matrix protein 1, which belongs to the rad21 family. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.



Images

016yCE6VdoMSS7S.jpg

 WB analysis of Rad21(GB111435).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02ehxgffTqgsMHQ.jpg

 ICC analysis of Rad21(GB111435)red.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111435-100
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