Product Information
Protein full name | Cyclin-dependent kinase 4 |
Synonyms | Cell division protein kinase 4, PSK-J3, CDK4, Crk3, Cyclin dependent kinase 4, MGC14458, p34 cdk4, PSK-J3, CMM3, cyclin-dependent |
Uniprot ID | P30285 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse CDK4 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cytoplasm, Nucleus, Membrane |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human, Rat | 1: 100-1: 200 | breast cancer, colon, ovary |
Background
CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression.
Images
| IHC analysis of CDK4(GB11238-2). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CDK4(GB11238-2). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CDK4(GB11238-2). Sample: Rat ovary, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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