Anti-MAD1 Rabbit pAb

Anti-MAD1 Rabbit pAb

Antibodies Oncology
AO-03-GB11643-100
GB11643100,MAD1,GB11643,11643,MAD1 mitotic arrest deficient like 1,mitotic arrest deficient 1 like 1,TP53I9,MAD-1,MAD1L1,MAD 1,PIG9,TXBP181,Mitotic spindle assembly checkpoint protein MAD1
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Product Information


Protein full name

Mitotic spindle assembly checkpoint protein MAD1

Synonyms

MAD1L1, PIG9, TP53I9, TXBP181, MAD1, MAD1 mitotic arrest deficient like 1, mitotic arrest deficient 1 like 1

Uniprot ID

Q9WTX8

Immunogen

Recombinant protein corresponding to Mouse MAD1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoskeleton



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 500-1: 3000 brain



Background

Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.



Images

01XshVOjtdmEGwK.jpg

 IHC analysis of MAD1(GB11643).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

021Fdbgx29Mi7KQ.jpg

 IHC analysis of MAD1(GB11643).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03jl1T26TY0XFgw.jpg

 IF analysis of MAD1(GB11643)red.
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11643-100
: 10 Produits
Hurry! only 10 items left in stock.

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