Anti-Rad51 Rabbit pAb
   

Anti-Rad51 Rabbit pAb

Antibodies Oncology
AO-03-GB11573-100
GB11573100,GB11573,HHsRad51,11573,HsT16930,Rad51,RAD51A,BRCC5,Rad-51,RECA,Rad 51,RAD51 recombinase,RAD51,FANCR,MRMV2,DNA repair protein RAD51 homolog 1
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399,00 €
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Product Information


Protein full name

DNA repair protein RAD51 homolog 1

Synonyms

RAD51, BRCC5, FANCR, HHsRad51, HsT16930, MRMV2, RAD51A, RECA, RAD51 recombinase

Uniprot ID

Q08297

Immunogen

Recombinant protein corresponding to Mouse Rad51

Isotype

IgG

Predicted MW./Observed MW.

37 kDa / 37 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoskeleton, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 thymus
IHC/IF Mouse, Rat 1: 500-1: 4000 brain, testis
ICC/IF Human, Mouse, Rat 1: 100-1: 400 HeLa



Background

RAD51 is a eukaryotic gene, which is highly conserved from yeast to humans. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks. In humans, RAD51 is a 339-amino acid protein that plays a major role in homologous recombination of DNA during double strand break repair. In this process, an ATP dependent DNA strand exchange takes place in which a template strand invades base-paired strands of homologous DNA molecules. RAD51 is involved in the search for homology and strand pairing stages of the process.



Images

01HxGbEGDupc49L.jpg

 WB analysis of Rad51(GB11573).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02JsZ4i31Zpvphq.jpg

 IHC analysis of Rad51(GB11573).
Sample: Mouse ovary, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03t3DDVdwrdHXcO.jpg

 IHC analysis of Rad51(GB11573).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04uzY0KBYfDGyUG.jpg

 IHC analysis of Rad51(GB11573).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05X7yAf9eRZvh9X.jpg

 IHC analysis of Rad51(GB11573).
Sample: Rat ovary, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06waGs2EAaBnEdH.jpg

 IHC analysis of Rad51(GB11573).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

07aTWvcgppCzvDo.jpg

 IF analysis of Rad51(GB11573)red.
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08wv14Yfl3JR7Zp.jpg

 IF analysis of Rad51(GB11573)red.
Sample: Rat parencephalon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09bc5pbi7hYgSri.jpg

 IF analysis of Rad51(GB11573)red.
Sample: Rat prefrontal lobe, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

10XTzRjMQ1AhKIA.jpg

 ICC analysis of Rad51(GB11573)red.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

11XOoc5i6cgBMef.jpg

 ICC analysis of Rad51(GB11573)red.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11573-100
: 10 Produits
Hurry! only 10 items left in stock.

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