Recombinant Anti-Raf1 antibody (Rabbit mAb)

Protein full name

RAF proto-oncogene serine/threonine-protein kinase

Synonyms

RAF1, Raf-1 proto-oncogene, Serine/threonine kinase, CMD1NN, CRAF, Proto-oncogene c-RAF, NS5, Raf-1, C-Raf, Raf1

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Raf1

Uniprot ID

P04049

Isotype

IgG

Clone Number

SB716

Purity

Affinity purification

Predicted MW./Observed MW.

73 kDa / 73 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1500

Hep G2, A549, PC-12

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. The c-Raf protein is part of the ERK1/2 pathway as a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras subfamily of membrane associated GTPases. C-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, from the TKL (Tyrosine-kinase-like) group of kinases.

Images

WB analysis of Raf1(GB15780).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.