Recombinant Anti-TMS1/ASC antibody (Rabbit mAb)

Protein full name

Apoptosis-associated speck-like protein containing a CARD

Synonyms

ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1

Immunogen

Recombinant protein corresponding to Mouse TMS1/ASC

Uniprot ID

Q9EPB4

Isotype

IgG

Clone Number

SB410

Purity

Affinity purification

Predicted MW. / Observed MW.

22 kDa / 22-25 kDa

Applications

Species

Dilution

Positive Tissue

WB

Mouse

1: 500-1: 1000

spleen, colon

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Images

WB analysis of TMS1/ASC(GB154054).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.