Recombinant Anti-Cyclophilin A antibody (Rabbit mAb)

Recombinant Anti-Cyclophilin A antibody (Rabbit mAb)

Antibodies Oncology
AO-03-GB151283-100
PPIase A,Cyclophilin A,Ppia,Cyclosporin A-binding protein,Rotamase A,SP18
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Product Information


Protein full name

Peptidyl-prolyl cis-trans isomerase A

Synonyms

PPIase A, Cyclophilin A, Ppia, Cyclosporin A-binding protein, Rotamase A, SP18

Uniprot ID

P17742

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclophilin A

Isotype

IgG

Clone Number

SB413

Predicted MW./Observed MW.

18 kDa / 18 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 HeLa, spleen, brain



Background

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.



Images

01LZcgL5FxAVJgX.jpg

 WB analysis of Cyclophilin A(GB151283).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

022JsxSYeStU1xO.jpg

 WB analysis of Cyclophilin A(GB151283).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB151283-100
: 10 Produits
Hurry! only 10 items left in stock.

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