Recombinant Anti-Bax antibody (HRP Conjugated)

Recombinant Anti-Bax antibody (HRP Conjugated)

Antibodies Oncology
AO-03-ZB15690-HRP-50
BAX,BCL2L4,BCL2 associated X protein,BCL2 associated X,Apoptosis regulator BAX,Bcl-2-Like Protein 4,Bcl2-L-4,Bax-protein, apoptosis regulator
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Ajouter souhaits

Product Information


Protein full name

Apoptosis regulator BAX

Synonyms

BAX, BCL2L4, BCL2 associated X protein, BCL2 associated X, Apoptosis regulator BAX, Bcl-2-Like Protein 4,Bcl2-L-4, Bax-protein,  apoptosis regulator

Uniprot ID

Q07813

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Bax

Predicted MW./Observed MW.

21 kDa / 21 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 293, Hep G2, kidney
Incubation condition After blocking, incubate the membrane with HRP conjugated Anti-Bax antibody(ZB15690-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.



Images

010vdMZRvTzlr1B.jpg

 WB analysis of Bax(ZB15690-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-Bax antibody 1: 500, 4℃ overnight.

02RAnmIvTApxOdL.jpg

 WB analysis of Bax(ZB15690-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-Bax antibody 1: 500, 4℃ overnight.

03HTvyz7scWKg57.jpg

 WB analysis of Bax(ZB15690-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-Bax antibody 1: 500, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-ZB15690-HRP-50
: 10 Produits
Hurry! only 10 items left in stock.

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