Recombinant Anti-TMS1/ASC antibody (Rabbit mAb)
   

Recombinant Anti-TMS1/ASC antibody (Rabbit mAb)

Antibodies Oncology
AO-03-GB155270-100
ASC,CARD5,hASC,PYCARD,PYD and CARD domain containing,TMS,TMS 1,TMS1,Apoptosis-associated speck-like protein containing a CARD
check En Stock
Hurry! only 10 items left in stock.
429,00 €
HT
Quantité

Ajouter souhaits

Product Information


Protein full name

Apoptosis-associated speck-like protein containing a CARD

Synonyms

ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1

Uniprot ID

Q9ULZ3

Immunogen

Recombinant protein corresponding to Human TMS1/ASC

Isotype

IgG, κ

Clone Number

SB252

Predicted MW./Observed MW.

22 kDa / 22-25 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm,Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 THP-1, MCF-7
IHC/IF Human 1: 500-1: 1500 ovarian cancer, colon, tonsil
ICC/IF                 



Background

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.



Images

01LMefjVqFjVn0G.jpg

 WB analysis of TMS1/ASC(GB155270).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

026Au7Zr10we7aa.jpg

 IHC analysis of TMS1/ASC(GB155270).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

03InmuFEId0CHcS.jpg

 IHC analysis of TMS1/ASC(GB155270).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

04B0Jyob133JVoa.jpg

 IHC analysis of TMS1/ASC(GB155270).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05YI8hZiaTmkLcw.jpg

 IHC analysis of TMS1/ASC(GB155270).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

06UiqycowNNrPAg.jpg

 IHC analysis of TMS1/ASC(GB155270).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

07Bj8xz6Ab2QA8e.jpg

 IHC analysis of TMS1/ASC(GB155270).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

08oekeZjZGPYEUg.jpg

 IF analysis of TMS1/ASC(GB155270)red.
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09Jm9fEFzXMUFx3.jpg

 IF analysis of TMS1/ASC(GB155270)red.
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

10rFMJWQyrZHWqn.jpg

 IF analysis of TMS1/ASC(GB155270)red.
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

11AStXfjxixEFZn.jpg

 IF analysis of TMS1/ASC(GB155270)red.
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

12q3L4NoAQ5r6uY.jpg

 IF analysis of TMS1/ASC(GB155270)red.
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB155270-100
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package