Anti-Cyclophilin A antibody (HRP conjugated)

Anti-Cyclophilin A antibody (HRP conjugated)

Antibodies Oncology
AO-03-ZB111283-HRP-100
PPIase A,Cyclophilin A,Ppia,Cyclosporin A-binding protein,Rotamase A,SP18,Peptidyl-prolyl cis-trans isomerase A
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Product Information


Protein full name

Peptidyl-prolyl cis-trans isomerase A

Synonyms

PPIase A, Cyclophilin A, Ppia, Cyclosporin A-binding protein, Rotamase A, SP18

Uniprot ID

P17742

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclophilin A

Predicted MW./Observed MW.

18 kDa / 18 kDa

Purity

Affinity purification

conjugation

HRP



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000HeLa, Jurkat, thymus
Incubation conditionAfter blocking, incubate the membrane with HRP conjugated Anti-Cyclophilin A antibody (ZB111283-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background


Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.



Images

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WB analysis of Cyclophilin A(ZB111283-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-Cyclophilin A antibody 1: 1000, 4℃ overnight.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. 


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-ZB111283-HRP-100
: 10 Produits
Hurry! only 10 items left in stock.

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