Anti-Cleaved-Caspase-3 Rabbit pAb

Protein full name

Caspase-3

Synonyms

CASP3, CPP32, CPP32B, SCA-1, caspase 3

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Caspase-3

Uniprot ID

P42574

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW./Observed MW.

32 kDa / 31/19/17 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

L6, C2C12

IHC/IF

Human, Mouse, Rat

1: 500-1: 2000

Colon, tonsil, colon cancer

ICC/IF

Human, Mouse, Rat

1: 500-1: 2000

L6, C2C12, HeLa

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity).

Images

WB analysis of Cleaved-Caspase-3(GB115733).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of Cleaved-Caspase-3(GB115733).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

ICC analysis of Cleaved-Caspase-3(GB115733)red.
Sample: C2C12 cell, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.