Anti-Cleaved Caspase-7 Rabbit pAb
   

Anti-Cleaved Caspase-7 Rabbit pAb

Antibodies Oncology
AO-03-GB115728-50
CASP7,MCH3,CASP-7,Apoptotic protease Mch-3,CMH-1,ICE-like apoptotic protease 3,casepase,Caspase-7,capase,c-caspase
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299,00 €
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Product Information


Protein full name

Caspase-7

Synonyms

CASP7, MCH3, CASP-7, Apoptotic protease Mch-3 , CMH-1, ICE-like apoptotic protease 3

Uniprot ID

P55210

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Cleaved Caspase-7

Isotype

IgG

Predicted MW./Observed MW.

34 kDa / 20/30 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Secreted



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 Jurkat/C2C12/L6 treated with Staurosporine
ICC/IF Human, Mouse, Rat 1: 200-1: 800 Hela/C2C12/L6 treated with Staurosporine



Background

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 30 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury. The protein is involved in the activation cascade of caspases responsible for apoptosis execution.



Images

01spObnunWfVVAK.jpg

 WB analysis of Cleaved Caspase-7(GB115728).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1:500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02tLQpyf9eLqam2.jpg

 WB analysis of Cleaved Caspase-7(GB115728).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1:500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03Of0ZC4K8Djx2c.jpg

 WB analysis of Cleaved Caspase-7(GB115728).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1:500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

044yTbnMEnQk4Hh.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: C2C12 cell, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05sVBrmPjDM2sR4.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: C2C12 cells with Staurosporine(1 μM, 3 hr), 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

062U40NlFvXuMaS.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: Hela cell, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07Am8HGwUF9zf00.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: Hela cells with Staurosporine(1 μM, 3 hr), 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

086pvK04pyzl8Rk.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: L6 cell, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09gYBnf40Hjf5XY.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: L6 cells with Staurosporine(1 μM, 3 hr), 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

10vyakzEUqEnNcm.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: C2C12 cell, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

11KaJ86Sh3Nv5V7.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: Hela cell, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

12SsZUEqFdYTvf1.jpg

 ICC analysis of Cleaved Caspase-7(GB115728)red.
Sample: L6 cell, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB115728-50
: 10 Produits
Hurry! only 10 items left in stock.

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