Product Information
|
Protein full name |
Caspase-7 |
|
Synonyms |
Apoptotic protease Mch 3, CASP-7, CASP7, Caspase 7, Caspase7, CMH-1, ICE-LAP3, ICE like apoptotic protease 3, MCH3 |
|
Uniprot ID |
P97864 |
|
Immunogen |
Recombinant protein corresponding to Mouse Caspase-7 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
34 kDa / 20/30/35 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | C2C12, L6, Jurkat |
Background
Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 30 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury. The protein is involved in the activation cascade of caspases responsible for apoptosis execution.
Images
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WB analysis of Caspase-7(GB115727). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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WB analysis of Caspase-7(GB115727). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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|
WB analysis of Caspase-7(GB115727). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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|
WB analysis of Caspase-7(GB115727). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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