Anti-MLKL Rabbit pAb

Anti-MLKL Rabbit pAb

Antibodies Oncology
AO-03-GB115699-50
Mixed lineage kinase domain like,Mixed lineage kinase domain-like protein,Mixed lineage kinase domain like protein,115699,MLKL_HUMAN,hMLKL,mixed lineage kinase domain like pseudokinase,GB115699,Mixed lineage kinase domain-like protein
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Ajouter souhaits

Product Information


Protein full name

Mixed lineage kinase domain-like protein

Synonyms

hMLKL, Mixed lineage kinase domain like, Mixed lineage kinase domain like protein, Mixed lineage kinase domain-like protein, mixed lineage kinase domain like pseudokinase

Uniprot ID

Q8NB16

Immunogen

KLH conjugated Synthetic peptide corresponding to Human MLKL

Isotype

IgG

Predicted MW./Observed MW.

54 kDa / 54 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Cell membrane, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 HeLa, HT-29, PC-3
IHC/IF Human 1: 500-1: 1000 liver cancer, tonsil



Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.



Images

01hBjY5surnRkN4.jpg

 WB analysis of MLKL(GB115699).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02jBSNVJH22x2B1.jpg

 IF analysis of MLKL(GB115699)red.
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

03UhyCm3pl4Mbwk.jpg

 IF analysis of MLKL(GB115699)red.
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB115699-50
: 10 Produits
Hurry! only 10 items left in stock.

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