Product Information
|
Protein full name |
BAG family molecular chaperone regulator 1 |
|
Synonyms |
BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46 |
|
Uniprot ID |
Q60739 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Bag1 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus, Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse, Rat | 1: 150-1: 600 | testis |
Background
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain of HSPA8/HSC70. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
Images
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IHC analysis of Bag1(GB115597). Sample: Mouse testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 150, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of Bag1(GB115597). Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 150, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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