Product Information
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Protein full name |
Receptor-interacting serine/threonine-protein kinase 3 |
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Synonyms |
Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3, Rip3 , KIAA0864, M RIP, MPRIP, MRIP, p116Rip, Rho interacting protein 3, RHOIP3 |
|
Uniprot ID |
Q9QZL0 |
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Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse RIP3 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
53 kDa / 53 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, cytosol, Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse | 1: 500-1: 1000 | L-929,RAW 264.7,N2A(low),A20 |
| ICC/IF | Mouse | 1: 500-1: 1000 | L929, NIH-3T3 |
Background
Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death.Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1.Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage.In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.
Images
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WB analysis of RIP3(GB115634). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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ICC analysis of RIP3(GB115634)red. Sample: L929, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
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ICC analysis of RIP3(GB115634)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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