Anti-TMS1/ASC Rabbit pAb
   

Anti-TMS1/ASC Rabbit pAb

Antibodies Oncology
AO-03-GB115270-50
PYD and CARD domain containing,ASC,GB115270,115270,hASC,TMS,TMS1,PYCARD,TMS1/ASC,CARD5,TMS 1,TMS-1,Apoptosis-associated speck-like protein containing a CARD
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Product Information


Protein full name

Apoptosis-associated speck-like protein containing a CARD

Synonyms

ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1

Uniprot ID

Q9ULZ3

Immunogen

KLH conjugated Synthetic peptide corresponding to Human TMS1/ASC

Isotype

IgG

Predicted MW./Observed MW.

22 kDa / 22-25 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 300-1: 600 lymph node
IHC/IF Human 1: 400-1: 800 tonsil, liver, colon



Background

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.



Images

01EwME5jDF7fBjK.jpg

 WB analysis of TMS1/ASC(GB115270).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02y5B0bo9mHiYmM.jpg

 IHC analysis of TMS1/ASC(GB115270).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03VhE2wU71PgC0F.jpg

 IHC analysis of TMS1/ASC(GB115270).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04ZcWs1sWajGRlh.jpg

 IHC analysis of TMS1/ASC(GB115270).
Sample: Human liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

054Zi9cmE0PGL58.jpg

 IHC analysis of TMS1/ASC(GB115270).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06Lo6lD7R5kxJu9.jpg

 IHC analysis of TMS1/ASC(GB115270).
Sample: Human liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

07GFdfsViM2jn9H.jpg

 IHC analysis of TMS1/ASC(GB115270).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

08OdCCRCy26HXvW.jpg

 IF analysis of TMS1/ASC(GB115270)red.
Sample: Human liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09IyCK13uu4xhIk.jpg

 IF analysis of TMS1/ASC(GB115270)red.
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB115270-50
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Hurry! only 10 items left in stock.

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