Anti-TMS1/ASC Rabbit pAb

Anti-TMS1/ASC Rabbit pAb

Antibodies Oncology
AO-03-GB113966-50
PYD and CARD domain containing,ASC,113966,GB113966,TMS 1,hASC,GB113966100,TMS,TMS1,PYCARD,TMS1/ASC,CARD5,TMS-1,PYD and CARD domain containing
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Product Information


Protein full name

Apoptosis-associated speck-like protein containing a CARD

Synonyms

ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1

Uniprot ID

Q9EPB4

Immunogen

Recombinant protein corresponding to Mouse TMS1/ASC

Isotype

IgG

Predicted MW./Observed MW.

22 kDa / 22-25 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Mouse 1: 500-1: 1500 spleen, lymph node, small intestine
IHC/IF Mouse 1: 2000-1: 4000 small intestine, lung injury



Background

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.



Images

01MoiHsqQPuE6aK.jpg

 WB analysis of TMS1/ASC(GB113966).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02I770e8LPYdhO1.jpg

 IHC analysis of TMS1/ASC(GB113966).
Sample: Mouse Inflammatory model induced by lps(24h) small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03LFgb1xu1qxV6D.jpg

 IHC analysis of TMS1/ASC(GB113966).
Sample: Mouse lung injury, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04Um79HLVPhWVS1.jpg

 IHC analysis of TMS1/ASC(GB113966).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB113966-50
: 10 Produits
Hurry! only 10 items left in stock.

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